Bd facscanto ii measuring instruments quick reference manual. The bd facscanto ii system is a benchtop analyzer that delivers proven performance, accuracy, and high quality results. Provides streamlined software workflows for acquisition and analysis. Bd facscanto ii flow cytometer quick reference guide this guide contains instructions for both bd facscanto clinical and bd facsdiva software. Other software fcs and setup files created within bd facscanto clinical software can be imported into bd facsdiva software v5. Bd facscanto ii measuring instruments user manual manual pdf. In the fluidics system, the sample travels up the sample injection tube, and hydrodynamic focusing within the flow cell forces particles into a singlefile stream where laser light intercepts the stream at the sample interrogation point. The bd facscanto ii cuttingedge technology is built on more than three decades.
It automatically sets cytometer detector voltages and creates compensation tables based on a single run with bd facs 7color setup beads. Bd facscanto ii quick reference manual pdf download. The bd facscanto ii flow cytometry system delivers flow cytometry data on an efficient user friendly platform. Bd facsdiva software efficiently controls the setup, acquisition, and analysis of flow cytometry data from the bd facscanto ii workstation. Bd facs canto ii flow cytometry system gmi trusted. Waste tank is empty and refill the bottom layer of waste tank with bleach. Waiting allows the bd facsclean solution to dissolve deposits in the flow cell cuvette. The bd facscanto family of benchtop analyzers features reliable performance and accurate results for experiments requiring up to 10 parameters. A third violet 405nm, 30mw solid state laser can be added at an additional cost. Bd facscanto ii becton dickinson, san jose, ca, usa data file structure. Bd facscanto ii becton dickinson, san jose, ca, usa. Bd facscanto ii measuring instruments user manual manual. See headers and footers on page 100 in the bd facsdiva software reference manual. Facscanto ii standard operation protocol basic operation.
Bd facscanto ii flow cytometer technical specifications. Bd facscanto clinical software set up the cytometer with bd facscanto clinical software, even if you. It includes automated setup and compensation, applicationspecific analysis modules, and predefined reports with integrated quality control. Please read the full description by bidding on this item you accept the terms stated below. Accurate and reproducible enumeration of t, b, and nk. Interference there are no known incompatibilities with bd facscanto clinical. Multisite evaluation of the bd stem cell enumeration sce. Wellington, auckland, new zealand ec rep bd facsdiva software reference manual for version 8. Samples were analyzed on the bd facslyric system with bd facsuite clinical software, and on the bd facscanto ii system with bd facscanto clinical software and bd facs 7color setup beads. Check that the sheath cube is full and the waste tank is empty. Page 79 a dialog indicates bubble filter purge progress. Bd facscanto fluidics with bd facscanto clinical software or bd facsdiva software. In this guide, you will find a description of bd facsdiva software features specific to the hts option. One striking difference between the facscanto and older generation machines is that this system operates on a pcbased windows xp platform using bd facsdiva software.
The bd facsdiva software reference manual assumes you have a working knowledge of basic microsoft windows operation. The bd facscanto is often hailed as the new generation of flow cytometry. Becton dickinson pty ltd, 4 research park drive, macquarie university research park, north ryde nsw 21, australia becton dickinson limited, 8 pacific rise, mt. The bd facscanto ii provides a high degree of automation and quality control to reduce handson time for technicians and improves reliability of results. Bd facscanto ii the canto ii cant detect up to 10 fluorescent parameters with 2 scatter options and is capable of analyzing samples between 100 and 12,000 cells per second. Bd facsdiva software provides one of the most complete and robust feature sets available for flow cytometry. The cytometer uses facsdiva software for acquisition and analysis.
For equivalency between methods, data n 362 were analyzed with deming regression for absolute count and percentage of lymphocytes. At the heart of the cytometer, for example, the fluidics system features a fixedalignment flow cell to minimize startup time and improve reproducibility. To be politically correct, apart from miltenyi you should consider a couple of other manufacturers as well, such as sony, biorad, apogee. Bd facscanto flow cytometer w fluidics station cart. The bd facsaria ii was set for highspeed sorting 70 psi and 90 khz and both instruments were set up using bd cytometer setup and tracking software. Used flow cytometers bd facsaria, facscanto and accuri c6. Bd facscanto clinical software can import worklists from bd facs sample prep assistant spa software v2. Bd bioscience facscanto ii flow cytometer marshall. These tools assist in the ease and efficiency of using facscanto as they enable automated features.
As a leading provider of tools for flow cytometry, bd biosciences is committed to offering comprehensive, innovative software. Jun 07, 2006 the bd facscanto is often hailed as the new generation of flow cytometry. The bd facscanto ii system supports only two uses of the 2d barcode reader. Follow only those sections that apply to the software you are running. Bd lsr ii, bd facscanto, and bd facscanto ii flow cytometers modified with the hts can acquire samples from plates or tubes. Facscanto flow cytometer from bd biocompare product. Bd facscanto ii flow cytometry system becton dickinson. You will get everything in the photos, nothing more or less. Live chat now available monday through friday, 7am 8pm edt. Bd facsdiva software is for in vitro diagnostic ivd use when used with ivd reagents and cytometers. Bd facscanto ii canto 10color with canto clinical software. View online quick reference manual for bd facscanto ii measuring instruments or simply click download button to examine the bd facscanto ii guidelines offline on your desktop or laptop computer. The bd bioscience facscanto ii flow cytometer is a benchtop analyzer providing reliable and accurate results for experiments with up to 10 parameters.
Figure 11 bd facscanto ii flow cytometer the bd facscanto ii flow cytometer consists of a fluidics subsystem, an optics subsystem, and an electronics subsystem. Bd facsdiva operating software is common across many bd cell analyzers and cell sorters, including bd lsr and bd facsaria systems, giving researchers application flexibility. A fixed alignment flow cell, for example, minimizes startup time and. Firsttime users of bd facsdiva software should read. Please see photos for more information and serial numbers. Integration with workflow software and laboratory information systems to improve. It is the responsibility of the buyeruser to ensure that all added electronic files including software. If you are a current bd integrated diagnostic solutions customer seeking order status, technical, or application support please select from the following options. Bd facscanto clinical software is supplied with the bd facscanto and facscanto ii systems and includes application panels optimized for use with specific ivd reagent kits. A fixed alignment flow cell, for example, minimizes startup time and improves reproducibility. Running a basic 2 color flow cytometry experiment in bd facs diva. The loader is controlled by bd facscanto clinical software or bd facsdiva software.
Future growth with continued expansion of bd facscanto. Bd facscanto ii 422 configuration automated sample presentation bd facs universal loader bd facsloader with updated motor, pneumatic actuation, and sliding access doors software bd facsuite clinical software with 4 and 6color assay modules facscanto clinical software with multitest 6color and multitest 4color assays included as panels. Bd facscanto ii instructions for use manual pdf download. How to operate the bd facscanto flow cytometer preface dear colleague, the bd facscanto flow cytometer is use to operate, however, it is a delicate instrument for measuring fluorescence of single cells. Win32k patch was released with december 2019 security updates, crypto api patch was released with january 2020 security updates. This guide should help you operating the flow cytometer and leaving it such way that another colleague can use it another day.
Bd biosciences welcomes customer input on corrections and suggestions for improvement. Saves time, improves reproducibility high performance, innovative system the bd facscanto ii fluidics cart is designed to save time and improve system performance. Bd facsdiva software becton, dickinson and company. Using bd panels with bd facs spa and bd facscanto clinical software and the bd facslink lis interface. Its flexible modular design offers automation and up to eightcolor capacity for rapid and easy production of highquality data in clinical and research laboratories. Bd biosciences facscanto ii flow cytometer features. Bd facscanto ii is equipped with three excitation lasers wavelengths 405nm, 488nm and 640 nm. The bd facscanto ii system features many innovations. If you are not familiar with the windows operating system, refer to the documentation provided with your computer. The february 2020 security rollupcumulative update was released in february 2020. Aug 17, 2017 this video describes how to set up an experiment in facs diva version 8. Bd facscanto clinical software includes specific application modules optimized for use with specific ivd reagent kits.
Bd facscanto system 42 configuration with bd facscanto clinical software, k041074 bd facscanto system 42 configuration with bd facsdiva software, k040725 bd facscanto ii system 42 configuration with bd facscanto clinical software and bd facsdiva software, k062087 was also used a reference device. Facscanto ii standard operation protocol basic operation 1. Creating and working with experiment in bd facsdiva software a. Page 1 bd facscanto ii flow cytometer quick reference guide this guide contains instructions for both bd facscanto clinical and bd facsdiva software. The bd facscanto ii fluidics system is designed to streamline work, save time, and improve performance. This unit was just pulled from a laboratory but we can not properly test it. Running a basic 2 color flow cytometry experiment in bd. There is enough amount of sheath fluid to perform machine start up and sample acquisition. The kit is intended for in vitro diagnostic ivd use on either a bd facscalibur flow cytometer using bd cellquest v3. Bd facscanto ii or facsverse bc navios partec cyflow space thanks and best regards, pal jakso, phd biologist i would be interested what different answers you will get this time. Bd biosciences delivers software and workstations that are intended for running the instruments supplied by bd biosciences. Bd facscanto clinical software with spa ii sample prepper bd facscanto clinical software with spa iii sample prepper using bd panels with bd facscanto clinical software and the bd facslink lis interface.
Built on more than 25 years of bd experience and leadership in flow cytometry and multicolor analysis, the bd facscanto ii system is an easytouse benchtop analyzer that delivers proven performance, accuracy, and highquality results. Innovative designs for both the excitation optics and collection optics reduce excitation losses and dramatically improve collection efficiency. Download user manual manual of bd facscanto ii laboratory equipment, measuring instruments for free or view it online on. The canto ii cant detect up to 10 fluorescent parameters with 2 scatter options and is capable of analyzing samples between 100 and 12,000 cells per second. Running a basic 2 color flow cytometry experiment in bd facs. Cytometer function is controlled by bd facsdiva software. These panels feature automated gating, calculations, and report generation to deliver consistent, reproducible, and standardized analysis. Home products manufacturers bd biosciences bd facscanto ii products. Setup on page 1 in the bd facsdiva software reference manual. New worksheet features create headers and footers on the worksheet, including cytometer name, experiment name, user name, date and time, bd facsdiva software version, etc. Hours 247 for those who have been trained by our staff. Bd facscanto clinical software bd facsdiva software after the completion message displays, wait 5 minutes. Bd facscanto clinical software overview features workflow sample data productivity, consistency, and reliability from dedicated clinical software the ivd cleared bd facscanto ii system includes software designed to address the needs of todays busy clinical laboratory.
The modules feature automated gating, calculations, and report generation to deliver a consistent, reproducible, and standardized analysis. Designed for the bd facscanto and the bd facscanto ii flow cytometers, the software handles setup, acquisition, analysis, and automated loading of. A bd hlab27 software module for the bd facscanto ii system provides a complete system for rapid detection of hlab27 antigen, which is clinically relevant to the evaluation of seronegative spondyloarthropathies. Make sure the following steps are done before running your samples.
Accessgudid bd facscanto 00382906466037 bd facscanto system ii upgrade v2. Carousel report now includes the directory selected for each specimens data in the carousel. This video describes how to set up an experiment in facs diva version 8. Bd facscanto ii flow cytometer reference manual alfresco. Bd facscanto ii measuring instruments quick reference. Bd facscanto ii flow cytometer technical specifications subject. Accessgudid bd facscanto 00382906577696 facscanto ii 3.
Bd facscanto ii system lsrfortessa sample prep assistant systems facsymphony a3a5 systems product security patch bd kiestra tlawca octdec 2019 product security patch bd kiestra tlawca julyseptember 2019 product security patch bd kiestra tlawca april to june 2019. Cimi offers the bd facscanto ii system equipped with 3 lasers 405 nm, 488 nm, 633 nm that allow the use of up to 8 fluorochromes. The ivd cleared bd facscanto ii system includes software designed to address the needs of todays busy clinical laboratory. The back of the fluidics cart has some rust and some cosmetic damage. Even when acquiring samples using the hts, consult the. Sections are indicated with the appropriate software icons.
The bd facscanto ii flow cytometer is certified for clinical applications. The optics of the bd facscanto ii system consist of an excitation source with up to three lasers. Bd bioscience facscanto ii flow cytometer marshall scientific. Bd facscanto ii flow cytometer quick reference guide.
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